生物材料灾难恢复计划的最佳冰柜技术是什么？

自然灾害，扩大停电和设备故障会破坏无价的生物材料。

包括多个备份系统在内的灾难恢复计划对于所有生物库件和研究设施至关重要。在灾难场景中，冰柜类型的选择可以使完全样本丢失和维持生物材料完整性之间有所不同。

在过去,K飓风等自然灾害atrina (2005) and superstorm Sandy (2012) have caused severe damage to valuable collections of biomaterial. Inadvertent equipment failures have also jeopardized priceless tissue collections, exemplified by the dramatic loss of 1/3 of the world’s largest collection of autistic brain samples after freezer failure. These and similar unfortunate events have reshaped our thinking about disaster recovery and the need to protect stored biomaterial from events beyond our control.

Plans for risk mitigation and disaster recovery are now recognized to be an essential feature in biobanks, biotech companies, and core facilities at universities. However, one choice rarely considered in the context of disaster recovery is the selection of the type of freezers used for storage of cryogenic biomaterial. The consequences of this choice are highlighted in a newvideo来自Azenta Life Sciences，该公司的生物银行业务负责人Kathi Shea称重₂- 对机械冰柜的冷却储存技术。

She makes a compelling argument in favor of storage technologies based on liquid nitrogen.

机械冷却的冰柜需要压缩机的干燥环境，并需要24/7的功率。但是液氮冷却的冰柜可以长时间运行，而无需电力。”她解释说。

She points out that cryogenic storage in LN₂坦克具有其他冷藏技术无法提供的独特灾难缓解益处。

“In a power outage, full mechanical freezers will warm at a rate of up to 10°C per hour," she says, "Samples that are stored in an LN₂油箱要安全得多，因为这些储罐将使您的生物产品保持20天以上的温度。”

This extra time might make all the difference in disaster response scenarios. Emergency rescue operations, especially when properly planned, will (in all likelihood) be concluded within a few days. Biomaterial collections stored in LN₂如果适当维护并定期填充液氮，则基于温度波动或瞬态变暖事件（TWES）将不会受到损害。已知这些二聚体会导致干细胞的秋季生存能力和功能大幅下降。

在灾难恢复的情况下以及日常操作中，Azenta Life Sciences通过自动化为低温生物材料存储提供了理想的解决方案Biostore™III冷冻-190°C系统。

这些系统在灾难恢复中是有利的，因为它是LN₂- 基于LN后最多可以保持温度长达三个星期或更长时间₂补充。如果发生自然灾害或扩大的停电，则设备中的生物产品完全安全，并且在低于水的玻璃过渡温度（大约-135°C）的温度下存储，所有生物活动都扩散被认为停止了。

该系统非常适合日常操作，因为它可以最大程度地减少无辜样品的持续变暖事件的持续问题，例如恰好居住在靠近存储中检索的样品附近的样品。

实际上，LN₂坦克，整个架子通常用手工从冷环境中移除，以获取单个样品。在自动化中Biostore™III冷冻-190°C系统，仅检索相关的生物材料盒。

制定灾难恢复政策时，安装自动化的LN₂基于生物群和生物库以及需要在低温温度下存储生物样本的核心设施和生物实验室，基于生物群和生物库的冰柜系统将是一个理想的选择。

As Kathi Shea points out, “The BioStore™ III Cryo -190°C System retains the benefits of a manual LN₂freezer but also provides improved protection and efficiency with the use of sample automation. By having the automation, we provide consistency in sample handling. We minimize sample warmings, keeping all samples below the glass transition temperature and protecting the innocents.”

Naturally, planning for disaster recovery does not stop with the choice of the appropriate and best biostorage technology. Such plans should also include training sessions for key personnel and contingency plans for relocation of the entire lab.